Journal: International Journal of Molecular Sciences
Article Title: Lethal Arrhythmogenic Role of Left Ventricular Myocardial Interstitial Fibrosis in Apolipoprotein E/Low-Density Lipoprotein Receptor Double-Knockout Mice with Metabolic Dysfunction-Associated Steatohepatitis
doi: 10.3390/ijms26010144
Figure Lengend Snippet: The effects of a co-diet of ethanol and AD on hepatic fat accumulation, inflammation, and fibrosis. Examples of Oil-Red-O staining, CD68 immunostaining, and Sirius-Red staining of liver sections ( A ). The scale bars are 200 μm for Oil-Red-O and Sirius-Red staining and 30 μm for CD68 immunostaining. Representative results of hepatic Oil-Red-O content ( B ), CD68-positive area ( C ), Cd68 mRNA expression ( D ), Sirius-Red content ( E ), and Col 1a1 mRNA expression ( F ). The data are presented as mean values with standard deviations (SDs). Statistical significance (* p < 0.05 vs. WT+SCD, Et (−); † p < 0.05 vs. AL+SCD, EtOH (−); # p < 0.05 vs. AL+AD, EtOH (−)) was determined using ANOVA followed by Bonferroni–Dunn post hoc test.
Article Snippet: Immunofluorescence staining utilized specific primary antibodies, including CD68 (SCB sc-9139, rabbit polyclonal antibody, 1:200) for liver, alpha smooth muscle actin (α-SMA; #E2464, rabbit polyclonal antibody, 1:200; Spring Bioscience, Pleasanton, CA, USA), transforming growth factor-β1 (TGF-β1; SCB sc-31608, goat polyclonal antibody, 1:200), and tyrosine hydroxylase (TH; #NBP3-05555, chicken polyclonal antibody, 1:200; Novus Biologicals, Centennial, CO, USA) for LV.
Techniques: Staining, Immunostaining, Expressing